Shark cartilage preparation (SCP) was extracted from shark cartilage by the method of guanidine hydrochloride extraction, acetone fractional precipitation, and ultrafiltration. The effect of SCP on the cytoskeleton of endothelial cell was investigated by the whole cell scanning electron microscopy. The effects of SCP on endothelial cell migration and angiogenesis were measured by the cell migration experiment and the chicken embryo chorioallantoic membrane experiment, respectively. The results showed that the cytoskeleton of endothelial cell was wrinkled, and SCP inhibited the migration of endothelial cell, the inhibitory rate was concentration dependent. SCP also inhibited the angiogenesis in the chorioallantoic membrane of chicken embryos significantly. These results suggest that SCP has the inhibitory effect on angiogenesis, and the mechanism may be that SCP inhibits the cytoskeleton of endothelial cell, so inhibits the cell's migration, and so inhibits angiogenesis.