By using DNA electrophoresis, flow cytometry (FCM) analysis and laser confocal microscopy (LCM), the change of cell cycle and cytoskeleton of CNE-2Z cells induced by the inhibitors of protein kinase C (PKC) were observed. Cells were respectively treated by straurospine (ST) and sphingosine (SS) at the concentration of 1×10^-6 mol/L and 4×10^-5 mol/L, and cocultured for 24 hours. Ladders of DNA electrophoresis and hypodiploid peaks were discovered in the two treated groups. Compared with the control groups, the cell cycle percentage of S in treated groups by SS was much increased, but the percentage of G1 was significantly decreased (P＜0.05), and the percentage of G2 in ST treated groups was much increased, but both the percentage of S and G1 were much decreased (P＜0.01). The well-distributed chromatin DNA was seen in untreated cells, but fragmentary DNA in treated cells. The granuliform microfilament regularly lined along and formed the intact cell morphology in untreated group cells. The microflament clustered in disorder and formed clusters in induced cells. The results showed that the cell cycle and cytoskeleton may contribute to the CNE-2Z cells apoptosis induced by the inhibitors of protein kinase C.