The kinetic study of the incorporation of exogenous ganglioside GM3 into the lipid bilayer of rabbit sarcoplasmic reticulum (SR) was carried out by trace extraction of Gls and high performance TLC. The results showed that the amount of incorporation is related to GM3 concentration, incubation temperature and time. The optimum incorporation was observed when 8 μmol/L GM3 was incubated with SR vesicles for 90 min at 35℃. The obtained results suggested that the effect of exogenous ganglioside GM3 on SR membrane is not only a simple aqueous phase reaction, but also a kinetic course of the incorporation into the lipid bilayer of SR. Furthermore, the incorporation of GM3 can markedly increase the activity of SR Ca²⁺-ATPase. This provides basic experimental data to study the role of gangliosides in intracellular membranes.