Regeneration of liver after hepatec-tomy is a complex process. It has been thought that there might be some growth factors and genes which play important roles in this process. In order to understand the regulatory mechanism of liver regeneration at molecular level, the changes in gene expression of regenerating liver have been demonstrated by mRNA differential display. As a result, four candidate cDNA fragments were obtained, including one down-regulated and three up-regulated. DNA sequencing demonstrated that all of them were novel. These sequences have been assigned the database accession numbers in EMBL as below: X95721, X95722, X95723, X97973.