Rapid Purification of Trichosanthin by Blue Sepharose CL-6B
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摘要:
差光谱显示染料cibacron blue F3GA与天花粉蛋白(TCS)有特异性结合，复合物在可见光部分的最大吸收波长在690 nm，摩尔消光系数ε=2.6×10-3(mol/L)-1·cm-1,解离常数Kd=1.8 μmol/L，0.5 mol/L NaCl可使复合物解离.根据这一特点，用Blue-Sepharose CL-6B凝胶从栝篓块茎中亲和纯化了TCS.此法快速、简便、高效，易于大量制备.
Abstract:
It is revealed by difference spectra that cibacron blue F3GA could bind specifically to Trichosanthin(TCS). The spectral absorption maximum is on 690nm with molar absorption coefficient of 2.6×10-3 (mol/L)-1·cm-1. The dissociation constant is 1.8 μmol/L, and 0.5 mol/L NaCl could dissociate the complex. Based on these results, TCS is purified from the root tuber of Trichosanthes Kirilowii by Blue Sepharose CL-6B. This method is rapid, simple, efficient and could be applied in mass preparation of TCS.
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