通过偶联剂3-(2-吡啶联巯基)丙酸N-羟基琥珀亚胺酯(SPDP)及改变配料比, 合成了两个R-藻红蛋白(R-PE)与C-藻蓝蛋白(C-PC)的复合物A和B.利用吸收光谱确定了分子内R-PE与C-PC的摩尔比为6∶1和2∶1. 通过荧光光谱, 观察到能量传递现象, 并计算出能量传递效率为63%和88%.证明分子内能量传递效率很高. 二硫苏糖醇(DTT)还原连接R-PE与C-PC的二硫桥键后, 能量传递被阻断. 这一现象进一步证明复合物中存在分子内能量传递.
Two conjugates of R-phycoerythrin (R-PE) and C-phycocyanin (C-PC) were synthesized through a coupling agent, N-succinmidyl 3-(2-pyridyldithio)-propionate and the alternation of the ratio of R-PE to C-PC. Their ratio of R-PE to C-PC were determined by absorption spectra. One is 6∶1, the other is 2∶1. The phenomena of intramolecular energy transfer were characterized. The calculated results showed that the efficiency of energy transfer from R-PE to C-PC was 63% and 88% respectively. The efficiency of energy transfer is better. When the disulfide bridge between R-PE and C-PC was reduced by DTT, the energy transfer was stopped. The phenomena further verified the intramolecular energy transfer from R-PE to C-PC.
赵继全,赵井泉,杨紫萱,张建平,蒋丽金.藻胆蛋白复合物的合成及其分子内能量传递[J].生物化学与生物物理进展,1997,24(5):435-440
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