国家自然科学基金(39570235)和上海科学技术发展基金(95XD14002)资助.
中国江浙产短尾蝮蛇突触前神经毒素(β1-Agkistrodotoxin,β1-AgTx)经制备电泳被进一步纯化.经SDS-PAGE鉴定, 观察到天然β1-AgTx以及经电泳纯化获得的碱溶β1-AgTx-OH-成分在电泳图谱上出现的聚合谱带, 然而这些聚合谱带的含量似乎并不随时程的延长而明显地增强.另经双向电泳分析发现, 无论是天然的β1-AgTx或经电泳纯化获得的碱溶β1-AgTx-OH-成分均含有三个具有不同等电点(pI 7.0, 5.8和5.4)及不同含量比例的异构体谱带.天然β1-AgTx中三个异构体的含量比并不随时程延长而明显改变, 碱溶β1-AgTx-OH-成分中的三个异构体的含量比则随着时程的延长而发生显著的变化.
β-Agkistrodotoxin (β1-AgTx), a presynaptic neurotoxin obtained from the venom of snake Agkistrodon blomhoffii brevicaudus (Jiangshu and Zhejiang Province, China) was further purified by the method of preparative electrophoresis. The polymerism was found in the native component of β1-AgTx and the purified β1-AgTx-OH- which was solubilized in the slight basic solution (pH 7.5) by SDS-PAGE analysis, however, the amount of polymers seems to be stable observed in the different days. Moreover, by two-dimensional electrophoresis analysis, it was found that both components of β1-AgTx and β1-AgTx-OH- were composed of three isoforms (pI 7.0, 5.8 and 5.4) with different contents, and the rate of content of isoforms in the component of β1-AgTx-OH- was changeable observed in the different days, but not in the β1-AgTx.
任惠民,吉永华.β-Agkistrodotoxin的聚合与异构体观察[J].生物化学与生物物理进展,1997,24(6):521-525
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