Recombinant eukaryotic expression vector DNA containing β-galactosidase gene was injected directly into retina of normal and nerve transected frog Rana pipiens. β-Galactosidase in retina neurons was still identified in two weeks after transfection. Gene transfection to retina neurons is non-selectivity for any neuron. The transfected neurons are around the injection area. Comparing with the normal, the neurons transfected in retina of frog optic nerve transected distribute in a slightly wider area.