A brief introduction is given about the application of X-ray diffraction to the study of the dynamic process of the protein. Firstly, it gives some description about using traditional and Laue X-ray diffraction to study the dynamic process of a protein in a reaction taking place in a few minutes. Secondly, it is about reaction synchronization used to study the dynamic process of a protein in a few seconds. By choosing unmatchable substrate and unsuitable pH value, by choosing temperature and pH value jumping and by choosing metal ion and photochemical induction, enzyme reaction can be activated instantaneously.