MAR Library and Its Application in Eukaryotic Genome Mapping
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摘要:
基质结合区(matrix association regions，MAR) 是真核生物基因组中特有的一种参与基因表达调控和染色体动力学的顺式作用元件，也是真核染色体上的一种固有且各不相同的分子标记.通过体外(in vitro)或体内(in vivo)结合法可以得到与核基质特异性结合的MAR分子而构建MAR文库.体外MAR作为一种结构性的边界元件可用于染色体物理图谱的构建；而体内MAR作为某一组织中与核基质特异性结合的功能元件，则可用于研究已知基因的表达调控和染色体组织以及对新基因的分析.
Abstract:
Matrix association region (MAR) is not only a kind of cis-acting element for regulation of eukaryotic gene expression and chromosome dynamics, but also a type of fixed and specific DNA marker on the eukaryotic chromosomes. MAR library can be constructed by in vitro or in vivo association of DNA fragments with the nuclear matrix. In vitro MARs can be used in construction of chromosome's physical map, and in vivo MARs can be applied in studying the expression, regulation and chromosomal organization of known genes, and analyzing the newly discovered genes.
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