The transfection of Cos-7, Vero and Namalwa cell lines by lipofection and electroporation methods have been examined. The lipofection method was found to be better than electroporation method in terms of higher transfection and convenient use. The electroporation method is superior to the lipofection method in terms of average transfection efficiency for a wider variety of cells lines. It was found that Namalwa cells could only be transfected by electroporation method, while Cos-7 and Vero cells could be transfected by both methods.