The Immuno-PCR antigen detection system has been developed for several years as a modefication of the ELISA method. But only a few of laboratorise applied this high sensitive quantitative assay, since it needs special apparutus or complicated operations.A new procedure of Immuno-PCR was established, which coats antibody or antigien on PCR tubes with 0.8% glutaraldehyde instead of on the wells of titer plates with alkine carbonate buffer. The assay could be coducted with simple handlings and ordinary apparutus, and a high sensitivity is obtained up to 10⁵ times higher than that of ELISA in S-100 antigen detection.