Based on molecular docking method, the influence of interaction energy and molecular interaction between mutated ligand combining functional domain “WSXWS” of human interleukin-6 receptor(hIL-6R) and human interleukin-6(hIL-6) is studied. The conformation changes on key amino acids of human interleukin-6 receptor when combining with human interleukin-6 and the interactions between human interleukin-6 receptor and human interleukin-6 are analyzed.