Three saporins,SO3a, SO3b and SO6,were separated and purified from the seeds extracts of Saponaria officinalis.The molecular weights of SO3a and SO3b are about 22 500,19 400,respectively,and their pI values are about 8.4 and the amino acid components are analysed.The contents of the secondary structure of SO3a and SO3b are determined by laser Raman spectrum.In the experiment it was also found by reverse phase capillary chromatography that SO6 which was reported by stripe to be a purified protein,contain two components in fact.