FEN-1(flap endo/exonuclease)是一种结构特异性核酸酶,它能识别特定的DNA分叉结构,并切除含有游离5′端的单链核酸. 在DNA复制过程中,FEN-1通过其外切酶、内切酶活力去除了冈崎片段前端RNA引物的最后一个核糖核苷.在DNA修复中,FEN-1以其内切酶活力参与了损伤碱基的修复过程.FEN-1基因含有两个保守区和一个PCNA结合区.
FEN-1 is a kind of structure-specsific nuclease, which could recognize specific DNA bifurcated structure and cleave single-strand DNA with free 5′ end. During DNA replication, FEN-1 5′→3′ exonuclease removes the last nucleoside of RNA primer attached to an Okazaki fragment. In DNA repair, FEN-1 5′→3′ endonuclease is involved in the process of removing the damaged nucleotide. FEN-1 gene contains two conserved regions and one PCNA binding region.
石斌山,余应年.结构特异性核酸酶FEN-1的功能和结构[J].生物化学与生物物理进展,2000,27(2):143-146
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