在实践基础上摸索出一种快速并有利于蛋白质回收的SDS-聚丙烯酰胺凝胶电泳染色-脱色方法:经常用的考马斯亮蓝R-250染色液短暂染色后直接用250 mmol/L KCl溶液脱色.这种方法染色-脱色的清晰程度与常规的染色-脱色方法接近,而且能使蛋白质回收率提高三倍多.
A fast staining-destaining method for SDS-PAGE which favours the recovery of proteins was developed:brief staining by Coomassie Brillant Blue R-250 followed by destaining with 250 mmol/L KCl.The SDS-PAGE stained-destained by this method has almost the same clearance compared with the one stained-destained by the usual method,and the recovery of protein can be enhanced more than three times by this method.
康彬,童哲.一种利于蛋白质回收的快速SDS-聚丙烯酰胺凝胶电泳染色-脱色方法[J].生物化学与生物物理进展,2000,27(2):210-211
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