PCR is now one of the key procedure in the field of molecular biology, but too many things affect the PCR result. Sometimes, a lot of PCR optimization experiments must be done before satisfactory results are obtained. So an optimization project with minimum times of test is needed. Uniform Design is what can meet this requirement. For 244 bp fragment in human apo E gene, the optimization of the ampified conditions included concentration of Mg²⁺, concentration of DMSO, denature time, annealing temperature, elongation time, number of cycles based on Uniform Design was reported. The results show that a set of PCR conditions with specific, high yield amplified product can be obtained for purified template or simple-treated template by 6～10 times of optimization experiment. The Uniform Design is useful for the optimization of PCR conditions.