Y2 receptor, one of the two early recognized major receptor subtypes of neuropeptide Y (NPY) was considered to be involved in multiplicate biological and pathological functions induced by NPY. In order to make antibody against Y2 receptor and study the distribution of Y2 receptor, NPY Y2 receptor gene was amplified by RT-PCR from the total RNA of rat hippocampus. Then the C-terminal fragment of Y2 receptor was amplified by PCR and cloned into expression vector. The expression vector of C-terminal peptide of NPY Y2 receptor was transformed in E.coli, and the product of expression was purified.