中国科学院广州分院及广东省科学院测试基金和中国科学院“九五”重点基金(KZ952-J1-105)联合资助.
几种抗氧化剂的浓度与其清除1,1-二苯基苦基苯肼(DPPH)能力呈显著的线性相关.不同抗氧化剂清除DPPH能力差异明显.抗坏血酸与DPPH反应的灵敏性高于其抑制肾上腺素氧化的能力.用DPPH法和亚油酸氧化法同时测定了生长在不同光强下植物叶片抗氧化能力的变化,两种方法所得结论相一致.结果表明清除有机自由基法是一种快速、简便、灵敏的评估植物抗氧化能力的可行方法.
A very significant linear relationship was found between the capacity of scavenging DPPH free radical and concentrations of six antioxidants (r=0.898~0.994)determined by spectrophotometry.There was an obvious difference in the capacity of scavenging DPPH free radical among different antioxidants. Both scavenging DPPH and inhibiting the oxidation of adrenalin were closely related with the concentration of ascorbic acid. The change of DPPH levels is more sensitive than that of adrenalin in the presence of ascorbate. The antioxidative ability in leaves extracts of two woody plants grown under different light intensities was measured by either scavenging DPPH or inhibiting the oxidation of linoleic acid. The same conclusion was drawn through these two assays. It is suggested that scavenging DPPH free radical is a rapid,simple,sensitive and practical assay for the evaluation of antioxidative capacity in plants.
彭长连,陈少薇,林植芳,林桂珠.用清除有机自由基DPPH法评价植物抗氧化能力[J].生物化学与生物物理进展,2000,27(6):658-661
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