The current study was designed to reveal the preliminary function of NGX6 to NPC cells. In search of mechanisms of NGX6 2-D PAGE was used to identify proteins that were overexpressed in HNE cells that were transfected with NGX6. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry. Seven proteins such as Fas,ZNF and MHC-ⅡAg were identified. The possible significance of these findings is discussed. NGX6 may exert its effect on NPC cells by many ways.