采用铜离子活化海绵镉微型柱层析法将血清中的硝酸盐(NO-3)还原为亚硝酸盐(NO-2),再通过Griess反应测定NO-3/NO-2总量以反应体内一氧化氮(NO)水平,从而建立血清NO间接测定新方法. 结果表明,在pH 9.7 时,活化海绵镉还原NO-3能力强、速度快、抗干扰性好,还原率为96.4%~100.0%,检测范围为0~400 μmol·L-1,检测限为1.85 μmol·L-1,相对标准偏差为2.56%~3.46%,对NO-3的回收率为96.4%~102.2%,对NO-2的回收率为95.2%~101.3%,对混合标准液的回收率为98.7%~104.4%.该方法具有简便快速、灵敏准确、样品和试剂用量小等优点,可在临床推广应用.
A new assay for indirectly detecting nitric oxide in serum was established. Nitrate was reduced to nitrite by activated spongy cadmium with copper ion on a microscale chromatographic column, then the nitrite was measured by the Griess reaction. The cadmium column has good vitality, high speed and low interference for the reducing reaction at pH 9.7. The reducing rates are 96.4%~100.0%. The linear range of the assay is 0~400 μmol·L-1, the limit is 1.85 μmol·L-1. The RSDs are 2.56%~3.46%, and the recoveries are 96.4%~102.2%, 95.2%~101.3% and 98.7%~104.4% for NO-3, NO-2 and NO-3/NO-2 (95∶5) respectively. This method is easy, rapid and precise, needing only a small amount of sample and reagents, and can be used in clinical assay.
邓健,吕昌银,张建明,袁亚莉.活化海绵镉微型柱层析法测定血清中一氧化氮[J].生物化学与生物物理进展,2001,28(4):579-581
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