This work was supported by Ph.D Initiative Fund of Jinan University.
根据血栓溶解会引起吸光度下降的特性,在细胞培养板上制作小血栓,利用酶标仪同时检测多个样品的吸光度变化.发现反应初期,血栓吸光度的降低同时间呈线性关系,其回归方程的斜率k与酶活性成正比.因此可用k来表示酶活性.此方法与蛋白凝块溶解时间法(CLT法)相关性很好.该法省时,成本低,是一种较好的、实用的检测溶血栓酶活性的方法.
Based on the decline of A630 with the clot lyses, the -A630 of many samples was detected at the same time with EL311 Auto Reader. It was found that the A630 declined linearly with time, and k under different dillutions could reflect the fibrinolytic activity of enzyme. This method costs less time, less reagent and correlated with CLT very well, indicating it was a good and useful method for determination of fibrinolytic enzymes.
谢秋玲,林剑,张玲.溶血栓酶活性测定的一种新方法[J].生物化学与生物物理进展,2001,28(4):582-583
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