Two modified one-step acid guanidinium thiocyanate-phenol methods were reported for the isolation of total cellular RNA from mineralized skeletal tissues. The measurement of optical density of RNA extracts showed that the ratios of A₂₆₀ to A₂₈₀ were greater than 1.85, the values of A₂₃₀ decreased to an acceptable level which meant the contaminated carbohydrate was removed mostly from the preparations of total RNA. Bands of 28S RNA and 18S RNA could be seen apparently on the 1 % formaldehyde-agarose gel. Total RNA could be reverse transcripted into cDNA and β-actin and BMP-2 gene were successfully amplified by PCR. The results showed that the total bone tissue RNA was the qualified contemplate for RT-PCR.