Using RACE-PCR technique, a full-length cDNA was cloned from Momordica charantia and named mocPHGPX. The entire mocPHGPX cDNA (AF346906) is 927 bp, and encodes 167 amino acids. The derived amino acid sequence data showed that this protein contains the typical (high conservative) structure of phospholipid hydroperoxide glutathione peroxidase (PHGPX) previously found in animals and plants. Northern blot analysis displayed that the corresponding transcript is abundant in the leaves and stems but lower in the roots. These results will be helpful to make a thorough investigation of the function of plant PHGPX and to understand the anti-oxidative systems of plants in an all-round way.