Tissue microdissection is potentially one of the most useful techniques in the comparative investigations of specific cells. A novel laser capture microdissection (LCM) has been developed to procure precisely the single cells from complex normal and diseased tissues, in a rapid and practical manner. In this technique, a transparent thermoplastil film(ethylene vinyl acetate polymer)is applied to the surface of the tissue section on a standard glass histopathology slide; a carbon dioxide laser pulse then specifically activates the film above the cells of interest. Strong focal adhesion allows selective procurement of the targeted cells. Now LCM system had been available in proteome analysis and is likely to have a profound impact on proteome analysis.