This work was supported by a grant from the National Natural Science Foundation of China(30080032).
酵母表面展示系统是继噬菌体展示技术创立后发展起来的真核展示系统,酵母的蛋白质折叠和分泌机制与哺乳动物细胞非常相似,对人的蛋白质表达和展示更具优越性.酵母细胞颗粒大,可用流式细胞仪进行筛选和分离.目前报道的两种酵母展示系统分别以α或a凝集素作为融合骨架.在蛋白质的定向进化、口服疫苗的研制等多方面均有报道.
Yeast surface display system had been developed greatly since the phage display was invented. It is well suitable for displaying mammalian cell-surface and secreted proteins that require endoplasmic reticulum-specific post translational processing for efficient folding and activity. Yeast cells are large enough particles, unlike phage, that can be screened and separated using flow cytometer. Yeast adhesion receptor, a agglutinin or α agglutinin, have been used as a surface display scaffold. Yeast surface display has been successfully applied in protein directed evolution and alive oral vaccines.
郭波,谢佩蓉,邹强,郑萍.酵母表面展示系统研究进展[J].生物化学与生物物理进展,2002,29(1):19-22
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