This work was supported by a grant from the National Natural Science Foundation of China (29971012).
用Fura-2显微荧光测钙技术,研究了用外源性一氧化氮(NO)供体S-亚硝基谷胱甘肽(GSNO)诱导的,人脐静脉内皮细胞系ECV-304细胞胞内游离钙离子浓度([Ca2+]i )升高以及硒的抑制效应.结果表明,GSNO作用于ECV-304细胞,短时间内即可导致其胞内游离钙离子浓度升高.胞外液换为无钙液或向胞外液中加入CdCl2(1 mmol/L)对GSNO引起的[Ca2+]i升高无影响.提示,GSNO刺激主要引起胞内钙库释放.而且,一氧化氮清除剂血红蛋白(Hb)对这一过程有抑制作用,说明GSNO引起的胞内钙库释放由NO介导.经亚硒酸钠(1 μmol/L)处理的细胞,其NO引起的[Ca2+]i升高幅度明显被抑制,说明NO的这种作用可能与细胞的氧化还原状态有关.
The NO-induced intracellular free calcium concentration ([Ca2+]i) rise and the inhibiting effect of selenium on this process were determined by using Fura-2/AM fluorescence measurement method in a human umbilical vein endothelial cell line: ECV-304. Experimental results indicated that there was a fast rise of [Ca2+]i by the treatment of S-nitrosoglutathione(GSNO), a donor of NO. The rise of [Ca2+]i was inhibited by Hb, a scavenger of NO. These results suggested that this rise of [Ca2+]i was induced by NO. There was no effect on [Ca2+]i response to NO by removing calcium ion from bath, or by adding the non-selective calcium channel antagonist, CdCl2(1 mmol/L)to the bath. When the cells were pretreated by sodium selenite(1 μmol/L),the rise of [Ca2+]i induced by NO was obviously inhibited. This result suggested that selenium can inhibit calcium store release.
邓英,黄开勋,徐辉碧.硒对NO诱导的内皮细胞内游离钙离子浓度变化的影响[J].生物化学与生物物理进展,2002,29(2):252-256
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