A new type of functional material: aminated silica gel was prepared through the hydrolysis and polycondensation of precursors tetramethoxy-silicane (TMOS) and γ-aminopropylmethyldimethoxysilane by sol-gel process.Glucose oxidase (GOD) was cross-linkly immobilized on the carrier aminated silica gel by cross-linking agent glutaraldehyde.The effects of immobilization conditions such as TMOS content，glutaraldehyde concentration，enzyme content given，temperature and pH on the activity of immobilized GOD were discussed in detail.The heat performance and storage stability of immobilized GOD were also investigated. The feasibility of GOD immobilized on the carrier was confirmed by the correlative infrared spectra.The optimum conditions were acquired as follows：TMOS content 10%，glutaraldehyde concentration 2.0%，enzyme content given 1 600 U，temperature 32℃ and pH 5.2.The immobilized GOD had high bioactivity.