The gene expression profiles of 1 176 genes were analyzed using Atlas^TM Mouse cDNA array 1.2 in Babl/c mice lung tissues during endotoxic shock. Results showed that the expression of 128 genes was up-regulated and 3 genes down-regulated at 2 hours after exposure to endotoxin; the expression of 51 genes was up-regulated and 21 genes down-regulated at 20 hours after exposure to endotoxin. The changes in gene expression were further confirmed by RT-PCR. The significance of above changes in gene expression was analyzed. The results would be helpful to elucidating the molecular mechanism of endotoxic shock.