公安部重点课题基金资助(998221403).
This work was supported by a grant from the Important Project Foundation of Ministry of Public Security (998221403).
为开展高效毛细管电泳技术对STR位点的检测,通过建立毛细管电泳-短小串联重复序列(STRs)多态性的检测方法,实现了利用毛细管电泳快速检测人凝血因子ⅩⅢB(F13B)STR位点PCR产物的长度多态性,结果F13B-STR基因型分型正确,图谱清晰.方法的建立可简化常规多态性检测,实现快速、准确分离.
The FⅩⅢB short tandem repeat(STR) alleles, four-base-pair difference in length, could be separated and identified by capillary gel electrophoresis(CGE).The gene types of these alleles were determined by runing with them a known-length DNA fragment. This study paved the way for gene typing of STR locus or individual identification by capillary electrophoresis.
裴黎,邓华,张丽君,姜成涛,赵兴春,常彩琴,王俭.毛细管电泳-短小串联重复序列多态性方法及对人凝血因子ⅩⅢB STR位点的检测[J].生物化学与生物物理进展,2002,29(3):460-463
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