To explore the cerebral cortex mechanism of visceral nociceptive sensation and its characteristics on the cell level with intracellular recording techniques，spontaneous biological electric activities and evoked responses of neurons in anterior cingulate gyrus（ACG） to stimulating ipsilateral greater splanchnic nerve（GSN） in 18 cats were investigated. Among 312 neurons，82 were visceral nociceptive neurons（VNNs），which mainly having five patterns of spontaneous biological electric activities. According to the characteristics of the evoked responses，VNNs were classified into specific visceral nociceptive neurons （SVNNs，92.68％） and non-specific visceral nociceptive neurons（NSVNNs，7.32％）. Modes of the evoked responses could be excitatory（65.86％），inhibitory（17.07％），or mixed ones（17.07％）.The results suggest that ACG may be one of the representative areas of the ipsilateral GSN afferent pathway，and there exist two kinds of VNNs in ACG，which may be differently involved in pain modulation. The results may provide new experimental data for specific theory of pain.