Human neuroglobin (NGB) is a newly-discovered gene functioned specifically in the oxygen supply and oxygen consumption in the brain. However, the full-length cDNA sequence of NGB was not reported yet. Using the in silicon sequence elongation technique, 5′-RACE and 3′-RACE technique, the full-length cDNA sequence of human neuroglobin was successfully obtained (GenBank accession number AF422797). The cDNA sequence of human NGB is 1 909 bp in size and capable of encoding a protein of 151 amino acids. The 5′-untranslated region is 375 bp, while the 3′-untranslated region is 1 078 bp including 27 bp of poly(A) sequence. Results demonstrated that the RACE technique coupled with electronic sequence elongation technique is useful to obtain unknown sequence of 5′-terminal or 3′-terminal part of a new gene.