To observe whether the re-expression of NAG7 gene could affect the gene expression of HNE1 cells, the cDNA microarray technique was employed to analyze the changes of gene expressions. 250 μg total RNA was extracted and 1 μg polyA mRNA was isolated. Reverse transcription was performed and cDNA probe was random-prime labeled with ³³P-dATP and hybridized with the cDNA microarray membrane containing 16 150 genes and ESTs. The hybridized result was confirmed by Northern blot analysis. FLA-3000A Plate scanner and Array Gauge software were used to screen and analyze the expressions of each gene. The background was eliminated and the differences of signal intensity of matched spots over 2 times were set as a marker to identify the differential expression genes. The results suggested that 179 genes were differential expressed, in which 91 genes were up-regulated and 88 genes were down-regulated in NAG7 re-expressed cells. These genes were involved in gene transcription, regulation, proliferation, metabolism, apoptosis, and so on. Northern blot result testified that growth arrest specific protein 1 (gas 1) expressed up-regulated. Especially, the previous results of proteomic research also found that the protein of gas 1 expressed up-regulated. Therefore, the data suggested that gas 1 gene plays a critical role in NAG7 re-expressed HNE1 cells, and provides an important clue to elucidate the mechanism of NAG7 gene.