Detection and analysis of differentially-displayed genes is a routine strategy for the study of different biological phenotype.A data-mining flowchart on the bioinformatic analysis of these genes is described,which includes sequence similarity analysis based on internet, contig assembly and full-length cDNA sequence obtaining.Moreover,the strategy for how to construct a local bioinformatic platform for large-scale analysis on novel genes is also introduced.All of this information will accelerate the progress on novel genes cloning and function prediction.