教育部留学回国人员科研启动基金(HG99003)和国家杰出青年科学基金(39925036)资助项目.
This work was supported by grants from The Ministry of Education of China (HG99003) and The National Science Fund for Distinguished Young Scholars (39925036).
以两种大、小亚基基因次序颠倒的重构型人caspase-8基因的pIRES2-EGFP真核表达载体转染HeLa细胞,用间接免疫荧光染色、免疫细胞化学染色和电镜观察等方法,研究重构型人caspase-8基因在HeLa细胞中的表达及其促凋亡活性.结果显示,两种重构型人caspase-8基因在HeLa细胞中的表达可以有效地诱导HeLa细胞凋亡.
The pIRES2-EGFP eukaryotic expression vectors carrying two kinds of reconstructed human caspase-8 genes with rearrangement subunits were transfected into HeLa cells. The expression and pro-apoptotic effects of reconstructed human caspase-8 gene on HeLa cells were analyzed by indirect fluorescent staining, immunohistochemical staining and electronic microscope etc.The results show that expression of the two human caspase-8 can induce HeLa cells apoptosis effectively.
桂俊豪,赵晶,许彦鸣,于翠娟,贾林涛,王成济,杨安钢.重构型人caspase-8基因的表达诱导HeLa细胞凋亡[J].生物化学与生物物理进展,2003,30(5):738-743
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