On the basis of T-Rex inducible expression system, a vector that express reporter gene and regulated gene simultaneously were constructed. The induction effects of tetracycline to the gene expression were studied in CV1 cells by transient transfection. An 8.9-fold increase in expression level of reporter gene was observed when CV1 cells, which were transiently transfected with the vector, were induced with tetracycline for 24 h, suggesting that the application of single-plasmid vecor in gene expression induction is also feasible.