In order to detect the distribution of silica-binding proteins in rice and other graminaceous plants, specific polyclonal antibodies against a silica-binding protein from rice, namely SBP117, are successfully raised by synthesized peptides which are conjugated with Keyhole Limpet Hemocyanin and used as antigens to immunize rabbits. Western blot and the immunoblot results indicate that the antibodies not only can react with silicon-binding proteins of rice, but also can cross react with the proteins of other silicon-accumulated graminaceous plants, while it does not react with the proteins of non-silicon accumulated dicotyledonous plants (such as tomato leaves) and BSA. These findings indicate that the homologous proteins of SBP117 are widely existed in the graminaceous plants. Furthermore, tissue printing study shows that SBP117 is mainly located at the epidermis of roots, shoots and leaves as well as in the vascular bundle of the rice roots and leaves. The distribution of SBP117 in rice plants is coincided with the sites of Si accumulation in rice reported previously. Therefore it is concluded that the silicon-binding protein (SBP117) may be involved in the control of silicon deposition in rice plants.