Extracellular fatty acid-binding protein (Ex-FABP) is another family member of fatty acid-binding protein, it regulates the metabolism of fatty acid, muscle fiber, bone and so on in chicken. Three single nucleotide polymorphisms(SNPs) were detected in 5′ regulatory region of Ex-FABP gene using PCR-SSCP and DNA sequencing in crossbreed chickens. The changes of the bonding-sites of transcription factor are due to a single point mutation T → C(－1011) and a C insertion mutation in genotype BB based on genotype AA, then results in a cap site lack, and increase four sites of a Nkx-2, a AhR/Ar and tow CF1. The relationship between SNPs and chicken meat traits was analyzed, the results showed that there was significant correlation (P<0.01) between genotype BB mutant and abdominal fat weight. The results of this study indicated that Ex-FABP gene is the main candidate gene that regulates abdominal fat traits in chicken.