It is a difficult problem in molecular biology study of nasopharyngeal carcinoma(NPC) to obtain pureness tumor cells from tiny NPC tissue.To seek a convenient and applied way to obtain pureness and high quality RNA from NPC tissue for cDNA Microarray ， the study was designed to use RNAlater technology to keep the NPC dissected tissue and obtain pureness NPC cells by microdissection and gain RNA through RNA line amplification technology. The results hinted that RNAlater technology could keep the stability of NPC tissue and obtain the pureness and high quality RNA for cDNA Microarray combined with microdissection and RNA line amplification through optimum conditions.