In order to comparatively analyze the core-fucosylated glycol-proteome expression profile of hepatocellular carcinoma cell lines (HCC) with different metastasis potentials, some key glycoprotein correlated with the metastasis of hepatoma were screened. Using SDS-PAGE, 2-DE followed by LCA lectin blot, differential display maps of core-fucosylated glycol-proteome were generated and analyzed, and then the glycoproteins were identified by a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF-MS/MS). 1-DE lectin blot showed that MHCC97-H and MHCC97-L, which were established by authors' institute and had higher metastasis potentials than Hep3B, displayed numerous bands. From the core-fucosylated glycol-proteomics expression profiles, (55±7) spots in Hep3B (n=3), (60±6) spots in MHCC97-L(n=3), and (61±4) spots in MHCC97-H (n=3) were detected. Matched with individual 2-DE maps, the core-fucosylated glycol-proteomics expression profiles showed that there were (25±3) detected spots in Hep3B(n=3), (30±4) detected spots in MHCC97-L and (28±3) detected spots in MHCC-97H (n=3). There were 13 unmatched spots between the expression profile of Hep3B and MHCC97-L, and 9 unmatched spots between Hep3B and MHCC97-H. The 12 differential glycoproteins were identified by MALDI-TOF-MS/MS. Individually differential core-fucosylated glycoproteomics expression profiles existed in HCC with different metastasis potentials. All the results suggested that the metastasis of HCC might be correlated with these differential glycoproteins.