The deduced amino acid sequence of the VER2 gene was analyzed in a computer. A peptide corresponding to 204～215 amino acids in VER2 sequence was synthesized by chemical method and purified using HPLC. The polypeptide was conjugated with BSA and the covalent complex was used as antigen to immunize rabbits. The antibody was then affinity purified before being used to probe VER2 protein in Paraplast sections. The immunolocalization results detected by the peptide antibody were consistent with that of the full length VER2 protein antibody. It is concluded that antibody prepared with synthetic peptide is credible and sensitive enough for immune labeling of proteins in paraplast sections. It is a sensitive and effective approach to combine preparation of antibody from synthetic peptide with paraplast section production for protein immunocytochemistry study.