An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and quantitate the Klebsiella pneumoniae (Kp) capsule glycoprotein. The rabbit antiserum of Klebsiella pneumoniae capsule glycoprotein was prepared successfully by using a purified Kp capsule glycoprotein as antigen and the antibody was purified with ammonium sulfate fractionation and adsorption. The sensitivity of the assay is 0.031 mg/L. The standard curve is linear from 2.5 mg/L to 30 mg/L. The error of inter-plate is from 1.04% to 3.22%, and the error of intra-plate is from 2.61% to 8.35%.