NDM-1, the cucumber mosaic virus containing a satellite RNA depresses the accumulation of helper viruses and attenuates the symptoms induced under field conditions. The kinetics of the accumulation of helper virus RNA and satRNA in infected tissues was studied in 4 different hosts. Dot blot hybridization was used in the quantitative analysis to compare the disciplinarian changing of viral RNA and satRNA at 5,10 and 40 d after inoculation. The analyses suggest that the relative loading of genomic RNA and satRNA of the CMV-DNM1 displayed a similar host- and time-effect trend but in different degree. The result in tomato plants is distinguishable from the other hosts: the loading of relative genomic RNA and satRNA ascended during the 5 d to 10 d post inoculation, and descended to the lowest at the 40 d. Also the NDM-1 in tomato tissue accumulated to the lowest levels among all the hosts. To disclose the relationship of the sat RNA and helper virus of NDM-1, the ratios of the accumulation of satRNA and genomic RNA in 4 hosts were compared, which shows that the highest ratio is 15.8,occurred at 40 d in tomato. Analyses suggest that the mechanism of resistance to viral RNA is that the accumulation of satRNA of NDM-1 reduces the yield of the accumulated viral RNA. The changing disciplinarians of NDM-1 in other 3 hosts showed few differences.