国家自然科学基金资助项目(90206041,30170297)和中国科学院创新方向性项目(KSCX2-SW214-1).
This work was supported by grants from The National Natural Sciences Foundation of China (90206041, 30170297) and The Key Foundation of The Chinese Academy of Sciences (KSCX2-SW214-1).
通过凝胶阻滞实验表明,人类神经tau能够与不同来源的DNA(λDNA, 质粒DNA以及PCR产物)相结合,形成tau-DNA复合物.每分子神经tau大约与长度为6~10 bp的核苷酸片段结合.原子力显微镜直接证实了tau与线性质粒DNA相结合形成串珠样的结构.采用单克隆抗体Tau-1,进行免疫组织化学的实验显示,神经tau不但弥散地分布在SY5Y细胞系的细胞质内,同时也存在于细胞核中,并形成染色致密的斑点.以上结果提示:神经tau在细胞核中可能参与了某种重要的生物学功能.
Electrophoretic migration shift assay shows that human neuronal tau can bind to λDNA, plasmid DNA and DNA fragment of PCR product from human cDNA. One mole of neuronal tau binds to a fragment of 6~10 bp nucleotides. Under atomic force microscope, a necklace complex of tau-DNA has been directly observed. Experiments in immunohistochemistry with a monoclonal antibody Tau-1 exhibit condense nuclear staining of tau as well as diffuse cytoplasmic staining of tau. It suggests that neuronal tau plays an important role in nucleus.
曲梅花,李辉,徐艳娟,赫荣乔.人神经tau蛋白与DNA相互作用[J].生物化学与生物物理进展,2004,31(10):918-923
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