In order to construct a fusion gene encoding hVEGF165 and fused hirudin(FH), which can not only accelerate endothelial cell proliferation but also inhibit thrombosis, the eukaryotic expression vector hVEGF165-FH/pcDNA3.0 was constructed which contained the hVEGF165 and fused hirudin(FH). Then, the constructed vector was transfected into endothelial cell strain(ECV304). The hVEGF165-FH fusion gene transcription and protein expression were examined by RT-PCR and Western blot. The activities of fusion hVEGF165-FH were identified by serials of endothelial cell proliferation tests and antithrombotic tests in vitro. The results showed that the fusion protein was successfully expressed and it had biological activities of both hVEGF165 and fused hirudin. This new fusion gene with multi-function might be a new weapon to prevent restenosis after percutaneous coronary intervention(PCI).