In order to investigate the immune protective efficacy and immune protective mechanism of the lipsome-encapsulated Helicobacter pylori (Hp) recombinant protein vaccines, the oral lipsome-encapsulated vaccines with/without CT were used. Phosphatidyl choline (PC) and cholesterols (Chol) were prepared using reverse evaporation method whose size distribution of folate liposomes was measured by electronic microscopy. BALB/c mice were divided into six groups and immunized by BPS alone, liposome alone, rUreB plus CT, liposome-encapsulated rUreB, liposome-encapsulated rUreB plus CT and liposome-encapsulated (rUreB and rKat) plus CT orally respectively once a week for four weeks. All mice were challenged by alive Hp three times in two weeks after the last immunization and sacrificed in five weeks after the last challenge. Hp was determined by the fast urease test. The bactrerial colonizing density semi-quantitation, the inflammation severity grades and activity grades of the gastric histopathology were observed. IFN-γ and IL-4 mRNA expression of spleen T lymphocyte cell in different groups were studied with RT-PCR. The lipsome-encapsulated vaccines were obtained successfully. The mean size of folate liposome was (0.7±0.2) μm. Protective rates of BPS alone, liposome alone, rUreB plus CT, liposome-encapsulated rUreB, liposome-encapsulated rUreB plus CT and liposome-encapsulated (rUreB and rKat) plus CT were 0(0/11), 0(0/11), 58.3%(7/12), 54.5%(6/11), 63.6%(7/11) and 75.0%(9/12) respectively. Protective rate of rUreB plus CT was equal that of the liposome-encapsulated rUreB group (P>0.05). Protective rate of liposome-encapsulated (rUreB and rKat) plus CT was significantly higher than that of liposome-encapsulated rUreB plus CT (P<0.05). The mice gastrically inoculated with the latter four immune antigens had been significantly less infected by Hp and had mild inflammation of gastric tissue (P<0.05). There was not significant difference among any vaccine group (P>0.05). After Hp attacked, the level of INF-γ mRNA expression of every vaccine group was significantly lower than that of the PBS and liposome group (P<0.05) while the level of IL-4 mRNA expression of every vaccine group was significant higher than that of the PBS and liposome group (P<0.05). There was not significant difference among any vaccine group (P>0.05). The lipsome-encapsulated vaccines can significantly decrease Hp coloning density and reduce inflammation degree in mouse model. The double- candidate vaccines have more stronger immune protective function against Hp than that of sigle-candidate vaccines. After Hp attack, the mice which infected without vaccine induce a predominant Th1 immune inflammatin response while the mice which have accepted vaccine can induce a predominant Th2 protective immune response.