Learning a new behavior is an exploration process that involves the formation and the modulation of association set between stimuli and neuronal networks' responses. To construct one appropriate learning model at networking level, on multi-electrode array, the closed-loop low frequency (1 Hz) pair stimulation was used to train the cultured hippocampal neuronal network and induce the learning behavior at networking level. Once the hippocampal neuronal network was trained successfully, the early postsynaptic responses were increased evidently, the response/stimulus ratio was increased and the response time decreased, furthermore, selective. These results indicate that the formation and the modulation of association between stimuli and neuronal networks' responses have been set, which means the learning behavior at networking level has occurred.