以胎牛为实验动物,应用免疫组化方法定性检测β-catenin在胎牛蹄边缘表皮和毛囊形态发生中表达的时空变化,探讨胎牛蹄边缘毛囊形态发生中β-catenin表达的时空变化规律. 结果显示:β-catenin在胎牛蹄边缘毛囊形态发生早期 (68~93天)表达于表皮、基底层、基板和毛芽中,其中基底层、基板、毛芽中呈强阳性,表皮中呈中阳性;在毛囊形态发生中期 (94~184天)表达于基底层、表皮和毛钉中,其中基底层、表皮、隆突区、内外根鞘和漏斗部呈中阳性;在毛囊形态发生晚期 (184~225天)只在表皮及基底层有表达,其中基底层呈弱阳性,表皮呈强阳性. β-catenin表达的时空变化规律提示,在胎牛蹄边缘毛囊的形态发生过程中β-catenin具有重要作用.
Temporal and spatial expressions of β-catenin investigated in the hair follicle and epidermis of the hoof periphery in bovine embryonic development. IHC (immunohistochemical method) was applied to qualitatively detect the temporal and spatial expressions of β-catenin. β-catenin was detected in suprabasal, epidermal basal layer, placode, hair bud in early phase(E68~93),and expressed strongly in epidermal basal layer, placode, and hair bud, in suprabasal expressed less strongly; in metaphase(E94~184), β-catenin was detected in epidermis, hair peg, and in suprabasal, epidermal basal layer, hair follicle bulge, inner root sheath, outer root sheath, follicular infundibulum expressed less strongly; in late phase(E184~225), β-catenin expressed weakly in epidermal basal layers, while expressed strongly in epidermal keratinocytes. The result suggested that β-catenin plays an important role in hair follicle morphogenesis in the periphery of bovine hoof in bovine embryos.
苏小舟,郁枫,赵云辉,吴晓梅,陈佳.β-catenin 在胎牛蹄边缘毛囊形态发生中表达的时空变化[J].生物化学与生物物理进展,2007,34(6):642-646
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