采用化学比色法测定正常皮肤(8例)、增生性瘢痕(10例)及瘢痕疙瘩(10例)组织中黄嘌呤氧化酶(xanthine oxidase,XO)、铜锌超氧化物歧化酶(copper,zinc-superoxide dismutase,CuZn-SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶 (glutathione peroxidase,GPX) 活性以及丙二醛(malonaldehyde,MDA)含量. 结果表明:与正常皮肤比较,病理性瘢痕中XO和CuZn-SOD活性增加、CAT活性降低(P < 0.05)而GPX活性不变,CAT/CuZn-SOD和GPX/CuZn-SOD活性比率下降(P < 0.05),同时MDA含量升高(P < 0.05). 增生性瘢痕、瘢痕疙瘩之间比较均无差异. 上述结果表明,在病理性瘢痕中,氧化酶XO,抗氧化酶CuZn-SOD、CAT以及GPX的活性改变可能是引起活性氧水平升高的原因之一,在抗氧化剂选择上,CAT可能较为合理.
In order to study the changes of main oxidase and antioxidase in the pathological scars, the tissues of hypertrophic scar(10 cases), keloid(10 cases) and normal skin(8 cases)were obtained. The concentration of malonaldehyde (MDA) and the activities of xanthine oxidase(XO), copper, zinc-superoxide dismutase(CuZn-SOD),catalase(CAT) as well as glutathione peroxidase(GPX) were detected by spectrophotometric method. Compared with normal skin tissues, the concentration of malonaldehyde and xanthine oxidase activity were significantly higer in pathological scars (P < 0.05), SOD activity were higher, CAT activity was decreased remarkably in pathological scars than that in normal skin tissues (P < 0.05). And the activity ratios of CAT/CuZn-SOD and GPX/CuZn-SOD were obviously reduced (P < 0.05). However, no significantly differences were observed in the decrease activity of GPX. Differences in above-mentioned indexes all were not remarkable between hypertrophic scars and keloids. These data suggest that the changes of XO, CuZn-SOD, CAT and GPX activities may be a reason resulted in an increase in the level of reactive oxygen species in pathological scars. In the alternative of antioxidants, CAT may be more reasonable.
李伟人,岑瑛,刘晓雪,李晓红,左凤琼.病理性瘢痕中主要氧化酶和抗氧化酶活性测定[J].生物化学与生物物理进展,2007,34(8):851-855
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