Kidney samples were obtained from db/db mice and their db/m littermates at the age of 4, 8, 12, 16, 20 and 24 weeks. The expression of ANGPTL2 was assessed by RT-PCR and immunohistochemistry, and then correlated with biochemical and histological indices of kidney injury. No significant difference of ANGPTL2 expression was found in the kidney of db/db mice and the control db/m mice at the age of 4 weeks, a time when all biochemical and histological indices indicated that the db/db mice were in pre-diabetic condition. However, with the development of obesity, hyperglycemia and proteinuria, the expression of ANGPTL2 in the kidney of db/db mice increased, which indicated that the increased expression of ANGPTL2 associated with diabetic nephropathy. ANGPTL2 protein was found distributed mainly in the glomerulus. It is along the capillary loop, located just outside the basement membrane, and in the same location as podocytes, which suggested that podocytes are the main origin of ANGPTL2 in the kidney. Besides, increased expression of ANGPTL2 was found in older db/m mice though it has not reached to a significant level, which may suggest that ANGPTL2 might have some thing to do with the kidney injury caused by ageing.